How to Use Peptides
From reconstitution to injection – everything you need to know, step by step.
What You’ll Need
Essential supplies before you begin
Peptide Vial
Your lyophilised (freeze-dried) peptide powder
BAC Water
Bacteriostatic water for reconstitution
Insulin Syringes
U-100 syringes (1ml or 0.5ml)
Alcohol Swabs
For sterilising vial tops and injection sites
Step-by-Step Visual Guide
A complete visual guide to preparing, reconstituting, injecting, and storing your peptides safely. Follow each step in order.
Preparation
Gather your supplies: peptide vial, bacteriostatic water, syringes, alcohol swabs, cotton ball, and bandage.
Remove the plastic caps from both vials to expose the rubber stoppers.
Wipe the top of each vial with an alcohol swab. Let air-dry.
Reconstitution
Draw the required amount of bacteriostatic water into the syringe. Use our calculator if unsure.
Inject the water slowly into the peptide vial, letting it run down the inside wall. Do not spray directly onto the powder.
Gently swirl the vial until the powder dissolves. Do not shake.
Check the solution: no undissolved particles, no bubbles, clear liquid. If not, wait and swirl again.
Draw your dose slowly from the reconstituted peptide vial.
Injection
Hold the syringe needle-up, flick to move air bubbles to the top, and push the plunger until a small drop appears.
Choose your injection site: belly fat around the navel or outer thigh. Rotate sites between injections.
Clean the injection site with an alcohol swab.
Pinch a fold of skin at the injection site to lift the subcutaneous fatty tissue away from the muscle.
Insert the needle at a 45° angle into the subcutaneous fatty tissue, usually approximately half the needle length. Do not inject into muscle. Inject slowly.
Aftercare, Storage & Disposal
Withdraw the needle and press a cotton ball gently on the site for a few seconds.
Apply a small bandage.
Dispose of the needle in a sharps container.
Store reconstituted peptides in the fridge (2-8 °C). Avoid freezing and direct sunlight. Use within 90 days.
Measuring Your Dose
Getting the right amount every time
Calculate Your Concentration
Your concentration = peptide (mg) ÷ water (ml). For example, 5mg peptide + 2ml water = 2.5mg/ml. Use our calculator for instant results
Convert Dose to Syringe Units
On a U-100 insulin syringe: 100 units = 1ml. If your dose is 0.1ml, that’s 10 units (10 tick marks) on the syringe. The calculator will show you the exact number of units for your desired dose.
Draw from the Vial
Insert the syringe needle into the vial through the rubber stopper. Turn the vial upside down. Pull the plunger to draw your calculated amount. Tap the syringe gently to move any air bubbles to the top, then push the plunger slightly to expel them.
Pro Tip
If you're using a 0.5ml syringe instead of 1ml, the markings are more spaced out, making it easier to measure small doses accurately. Recommended for peptides dosed under 300mcg.
Storage Guidelines
Protect your investment – proper storage preserves potency
Before Reconstitution
- Refrigerator (2–8°C) is ideal
- Room temperature is OK for short periods
- Avoid direct sunlight and heat
- Can be stored for 12+ months sealed
After Reconstitution
- Must be refrigerated (2–8°C)
- Use within 28 days
- Never freeze reconstituted peptides
- Keep vial upright, away from light
Category-Specific Dosing Guides
Each peptide category has unique dosing considerations
Weight Loss Peptides (Semaglutide, Tirzepatide, Retatrutide)
Key principle: Always titrate – start low and increase gradually over weeks.
- Semaglutide: Start at 0.25mg weekly. Increase by 0.25mg every 4 weeks. Maintenance: 1–2.4mg/week.
- Tirzepatide: Start at 2.5mg weekly. Increase by 2.5mg every 4 weeks. Maintenance: 5–15mg/week.
- Retatrutide: Start at 1mg weekly. Titrate based on tolerance. Being a triple agonist, expect stronger effects.
- Inject once weekly, same day each week. Subcutaneous in the abdomen.
- Take on an empty stomach or 30 minutes before a meal for best results.
Recovery Peptides (BPC-157, TB-500)
Key principle: Inject near the injury site for localised benefits, or subcutaneously for systemic effects.
- BPC-157: 250-500mcg daily, split into AM/PM doses for sustained healing. 4-8 week cycles.
- TB-500: Loading phase of 2-5mg twice weekly for 4 weeks, then 2mg weekly maintenance.
- The BPC-157 + TB-500 stack is the most popular healing combination.
- For gut healing, inject subcutaneously in the abdominal area.
Anti-Aging Peptides (Epitalon, GHK-Cu, MOTS-c)
Key principle: Cyclical use with rest periods mimics natural peptide release patterns.
- Epitalon: 5-10mg daily for 10–20 day courses, 2–3 times per year.
- GHK-Cu: 1-2mg daily subcutaneously, or topically for skin benefits. 4-8 week cycles.
- MOTS-c: 5-10mg weekly. Works synergistically with regular exercise.
Cognitive Peptides (Semax, Selank)
Key principle: Intranasal administration for rapid CNS delivery.
- Semax: 200-600mcg daily via nasal spray. Effects felt within 15-30 minutes.
- Selank: 200-600mcg daily via nasal spray. Non-sedating anxiolytic.
- Semax + Selank stack provides focus (Semax) + calm (Selank) simultaneously.
- 2–4 week cycles with equal rest periods.
Additional Categories
- DSIP (Sleep): 100–300mcg 30-60 min before bedtime. Subcutaneous. 2-4 week cycles.
- Melanotan II (Tanning): 250-500mcg daily loading for 2-4 weeks, then as needed before UV exposure.
- PT-141 (Sexual Health): 1-2mg as needed, 30-60 min before activity. Do not use more than once per 24 hours.